Figure 3. The Sema7a^R145W mutation increases hepatic FA and TG concentrations in mouse livers.

Male WT and Sema7a^R145W homozygous (HO) mice at 10 weeks old (n = 4 per group) were euthanized and their liver samples were prepared. (A) Gas chromatography tandem mass spectrometry (GC/MS) analysis of total saturated fatty acid (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) levels (μg/g of mouse liver) in WT and Sema7a^R145W homozygous mouse livers. FFA, free fatty acid. (B) Quantification of hepatic long chain FA (FA μg/g of mouse liver) in WT and Sema7a^R145W homozygous mice. (C) Score scatterplot corresponding to a principal component analysis of the lipidomic data in the livers of WT and Sema7a^R145W homozygous mice. (D) Quantitative analysis of lipid ion (μg/g of mouse liver) in WT and Sema7a^R145W homozygous mouse livers. (E) Heatmap analysis of the TG number of carbons and double bond contents in the livers of WT and Sema7a^R145W homozygous mice. (F) Proteomic analysis in the livers of WT and Sema7a^R145W heterozygous and homozygous mice (n = 5 per group). (G) Volcano plot of the quantified proteins from WT and Sema7a^R145W homozygous mouse livers (n = 5 per group). (H) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the differentially expressed genes in the pathways between WT and Sema7a^R145W homozygous mice. The data were analyzed by independent-sample t test, 2-tailed. *P < 0.05 versus the WT mice.