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. 2022 Aug 8;7(15):e154113. doi: 10.1172/jci.insight.154113

Figure 4. The Sema7aR145W mutation increases FA and TG synthesis and FA uptake in mouse livers.

Figure 4

(A) Proteomic heatmap analysis of the NAFLD pathway in the livers of WT and Sema7aR145W homozygous mice. The liver extracts from the WT2 and 3, WT4 and 5, HO2 and 3, and HO4 and 5 male mice were combined for proteomics analysis. (B) The relative levels of mRNA transcripts of the genes for FA and TG synthetic enzymes, FA uptake transporters, and FA partitioning in WT mice (n = 6), Sema7aR145W heterozygous mice (n = 5), and Sema7aR145W homozygous mice (n = 7). (C) Western blot analysis of the relative levels of Acc1, Fasn, Scd1, Gpat, Lipin2, Cd36, Fatp5, Caveolin1, Plin3, Plin5, and Cpt1β protein expression in the livers of WT (n = 4), Sema7aR145W heterozygous (n = 5), and Sema7aR145W homozygous (n = 5) mice. The data were analyzed by 1-way ANOVA with Tukey’s post hoc tests or by Kruskal-Wallis test with Dunn’s post hoc test analysis. *P < 0.05 versus the WT mice, #P < 0.05 versus the Sema7aR145W heterozygous mice.