Skip to main content
. 2022 Aug 22;7(16):e160909. doi: 10.1172/jci.insight.160909

Figure 4. Evobrutinib interferes with the differentiation of T-bet+ class-switched memory subsets in naive B cell cultures.

Figure 4

(A) CD27– naive B cells were purified from healthy donor blood and cultured under Tfh-like conditions using IL-21 and CD40L-3T3 cells for 11 days (n = 7–8). IFN-γ and CpG were added with and without evobrutinib (Evo). (B) Proportions of in vitro–induced T-bet+ B cells, class-switched (IgM–IgD–) memory B cells, and antibody-secreting cells (CD38hiCD27hi; ASCs). (C) Histogram overlays and quantification of CD21 and CD11c expression (MFI) by these cultured B cells. Cultures were performed in 8 independent experiments, with 1 donor per experiment. Repeated measures 1-way ANOVA with Fisher’s least significant difference post hoc test were used. *P < 0.05, **P < 0.01, ****P < 0.0001.