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. 2022 Aug 22;7(16):e160909. doi: 10.1172/jci.insight.160909

Figure 5. CXCL10-mediated migration of CXCR3+ class-switched B cells through human brain endothelial monolayers is attenuated by evobrutinib.

Figure 5

(A) Histogram overlays and quantification of CXCR3 and CXCR4 expression (MFI) by B cells from 5 healthy blood donors after stimulation with IL-21, CD40L, IFN-γ, and CpG for 48 hours. Evobrutinib (Evo) was added to IFN-γ– and CpG-inducing conditions. Data were collected as described in the legend for Figure 3, C and D. (B–D) Purified CD27+ memory B cells from 6 healthy blood donors were assessed for their selective migration across human brain endothelial monolayers in vitro. The proportions of viable non–class-switched (IgM+CD27+; NCS), class-switched (IgM–CD27+; CS), and IgG1+ B cells were studied before and after migration to medium, CXCL12, and CXCL10 in the context of CXCR3 expression. These FACS data were obtained from 5 independent experiments, with 1–2 healthy donors per experiment. Data are presented as the mean ± SEM. Repeated measures 1-way ANOVA with Fisher’s least significant difference post hoc test was performed. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.