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. 2022 Aug 22;7(16):e156115. doi: 10.1172/jci.insight.156115

Figure 1. LCM of the FF, mature scar, and adjacent alveoli in a UIP/IPF specimen.

Figure 1

(A) FFPE specimens were serially sectioned at 5 μm and stained with pentachrome (top left) or H&E (the other 8 panels). Notice that pentachrome stains the FF (hallmark lesion in UIP/IPF) in the color blue (blue arrow), while the mature scar tissue appears yellow in color (yellow arrow). We individually captured the FF (left middle and lower panels), the mature scar tissue (mid-middle and lower panels), and the adjacent alveoli (right middle and lower panels) for MS preparation and analysis. (B) Similarly, a nonfibrotic control specimen showing microdissection of alveoli. Scale bar: 100 μm.