Figure 7. Acral skin is associated with altered expression of keratin genes.

(A) UMAP dimensionality reduction plots of single-cell RNA-Seq data. Single-cell RNA-Seq was performed on keratinocytes isolated from paired palm and trunk skin biopsies as described in Figure 6. (B) Basal and granular layer single-cell keratin expression. Each individual data point represents the number of reads that mapped to the indicated gene in a single basal or granular layer keratinocyte. (C) RNA-Seq was performed on whole-skin biopsies obtained from palm and trunk. Box-and-whisker plots of representative keratin genes differentially expressed in palm skin are shown. (D) Box-and-whisker plot of KRT9 and KRT10 expression in palm skin. Scatter plot of KRT9 versus KRT1 expression in individual granular layer palm keratinocytes. Each dot represents a single granular layer keratinocyte. Note the strong correlation between KRT1 and KRT9 within each cell. (E) Correlation scatter plot of KRT15 and KRT78 in cultured keratinocytes, where each data point represents a different primary keratinocyte culture.