Skip to main content
. 2022 Sep 9;8(36):eabp8655. doi: 10.1126/sciadv.abp8655

Fig. 1. Sequence-dependent reduction of mvRNA replication and induction of IFN-β promoter activation.

Fig. 1.

(A) Schematic of mvRNA formation via intramolecular template switching. This process has the potential to create novel RNA structures. Produced mvRNAs are bound by RIG-I, leading to the expression of innate immune responses. ppp, triphosphate. (B) IFN-β promoter activation following transfection of in vitro transcribed segment 5 mvRNAs of 47 or 76 nt in length. (C) Replication of model mvRNAs in HEK293T cells by the influenza virus A/WSN/33 (H1N1) RNA polymerase. RNA levels were analyzed by primer extension, and the ability of mvRNA replication to induce IFN-β promoter activity was analyzed using a luciferase-based IFN-β reporter assay. Nonspecific primer extension signals in (C) are indicated with *. (D) RT-qPCR analysis of IFN-β mRNA levels. Data from three biological repeats are shown. n.s., not significant.