| Cell line (Homo sapiens) |
HEK293A |
Invitrogen: https://www.thermofisher.com/order/catalog/product/R70507?SID=srch-hj-R705-07
|
Catalog no. R705-07 |
lot #1942007; certified mycoplasma free by supplier |
| Cell line (Spodoptera frugiperda) |
Sf9 |
Thermo Fisher Scientific: https://www.thermofisher.com/order/catalog/product/12659017
|
Catalog no. 12659017 |
lot 421973 mycoplasma tested at OHSU |
| Recombinant DNA reagent |
Zebrafish GlyRa1 subcloned in pcDNA3 vectorf |
https://doi.org/10.1016/j.cell.2021.01.026
|
Uniprot identifier: O93430
|
|
| Recombinant DNA reagent |
Zebrafish GlyRa1 subcloned in pFastBac Dual vector |
This paper |
Uniprot identifier: O93430
|
|
| Software, algorithm |
OriginPro software |
https://www.originlab.com/
|
Origin 2019b |
|
| Software |
Clampex software, Molecular Devices |
https://support.moleculardevices.com/s/article/Axon-pCLAMP-10-Electrophysiology-Data-Acquisition-Analysis-Software-Download-Page
|
Clampex 10.7 |
|
| Software |
DC-STATS |
Plested and Lape, 2020
|
|
Our lab; https://github.com/DCPROGS/DCSTATS/releases/tag/v.0.3.1-alpha
|
| Software |
CVFIT |
Lape, 2020b
|
|
https://github.com/DCPROGS/CVFIT/releases/tag/v1.0.0-alpha
|
| Software |
MotionCor2 |
https://doi.org/10.1038/nmeth.4193
|
RRID:SCR_016499
|
http://msg.ucsf.edu/em/software/motioncor2.html
|
| Software |
cryoSparc |
https://doi.org/10.1038/nmeth.4169
|
RRID:SCR_016501
|
https://cryosparc.com/
|
| Software |
Coot |
https://doi.org/10.1107/S0907444910007493
|
RRID:SCR_014222
|
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
| Software |
Phenix |
https://doi.org/10.1107/S2059798318006551
|
RRID:SCR_014224
|
https://www.phenix-online.org/
|
| Software |
Pymol |
PyMOL Molecular Graphics System, Schrodinger, LLC |
RRID:SCR_000305
|
http://www.pymol.org/
|
| Software |
UCSF ChimeraX |
https://doi.org/10.1002/pro.3235
|
RRID:SCR_015872
|
http://cgl.ucsf.edu/chimerax/
|
| Antibody |
anti-human CD235a-APC (mouse monoclonal) |
Thermo Fisher Scientific |
Cat#: 17-9987-42; RRID:AB_2043823
|
FACS (5 µl per test) |
| Recombinant DNA reagent |
PLKO-GFP (plasmid) |
This paper |
|
GFP version of pLKO.1-Puro |
| Recombinant DNA reagent |
PLKO.1-Puro (plasmid) |
Sigma-Aldrich |
RRID:Addgene_10878
|
Pol III based shRNA backbone |
| Sequence-based reagent |
Gipc1_F |
This paper |
PCR primers |
GGGAAAGGACAAAAGGAACCC
|
| Sequence-based reagent |
Gipc1_R |
This paper |
PCR primers |
CAGGGCATTTGCACCCCATGCC
|
| Sequence-based reagent |
siRNA: nontargetin control |
Thermo Fisher Scientific |
4390843 |
Silencer Select |
| Peptide, recombinant protein |
Streptavidin |
Thermo Fisher Scientific |
Cat#: 434302 |
|
| Commercial assay or kit |
In-Fusion HD Cloning |
Clontech |
Clontech: 639647 |
|
| Chemical compound, drug |
CBR-5884 |
Sigma-Aldrich |
SML1656 |
|
| Chemical compound, drug |
SL30010 (SMALP 30010P) |
Polyscope |
|
http://polyscope.eu/markets/polyscience/
|
