Fig. 4.

A) t-SNE visualization of CD8 T cells using single-cell transcriptomics. Graph-based clustering was utilized to define the number of clusters and cluster membership in an unsupervised manor. Cells from subjects with no infection (blue) and with infection (pink) are indicated, with darkness of the blue and pink respectively representing the 24 identified clusters. B) Number of cells in each cluster for controllers and non-controllers. Upregulated transcripts per cluster. Proportion of cells per cluster expressing a given transcript (colored blue/pink) and proportion of cells outside each cluster expressing a given transcripts (colored grey) are indicated. Clusters with increased abundance in controllers (blue) and non-controllers (pink) respectively are shown. C) Normalized expression of key transcripts distinguishing clusters of CD8 T cells. Normalized expression of transcripts projected onto the previously described t-SNE visualization of CD8 T cells, ranging from high expression (dark yellow) to low (light yellow) or no expression (grey).