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Degradation of alginate trisaccharide by cytoplasmic enzyme fraction. Alginate trisaccharide (10 μg) was incubated with the enzyme fraction (5.6 μg of protein) for 15 min (lane 3), 1 h (lane 4), and 24 h (lane 5), and the products were analyzed by TLC. Tri, Di, and Mono indicate the tri-, di-, and monosaccharides generated from alginate, respectively. Lane 1, alginate disaccharide (10 μg); lane 2, alginate trisaccharide (10 μg).
