FIG. 4.

Effects of pH (A) and temperature (B) on activity of oligoalginate lyase. Experiments were carried out at 30°C using alginate trisaccharide (10 μg) and purified enzyme (1.8 mU). (A) To determine the effect of pH, reactions were performed at 30°C for 30 min in the following 50 mM buffers; sodium acetate (□), potassium phosphate (○), and Tris-HCl (▵). Activity at pH 8.0 in Tris-HCl buffer was set at 100%. (B) To determine the optimal temperature, reactions were performed for 30 min at various temperatures in 50 mM Tris-HCl buffer, pH 8.0. Activity at 37°C was set at 100%.