FIG. 5.

Substrate specificity of oligoalginate lyase. (A) Various substrates (0.5%) indicated on the top were incubated with oligoalginate lyase (1.8 mU) at 30°C, and products formed after 0- and 30-min incubations were analyzed by TLC. Tri, alginate trisaccharide; di, alginate disaccharide. (B) Alginate (0.5%) was incubated with oligoalginate lyase (1.8 mU) at 30°C for several hours, followed by TLC analysis. Lane 1, alginate trisaccharide (10 μg); lane 2, alginate disaccharide (10 μg). Tri, di, and mono indicate the tri-, di-, and monosaccharides from alginate, respectively.