FIG. 6.

Mode of action of oligoalginate lyase. Alginate trisaccharide (10 μg) was incubated with oligoalginate lyase (1.8 mU) for 30 min at 30°C, and the products were analyzed on TLC plates (lanes 3 in panels A and B) by staining with sulfuric acid (A) or TBA (B). Lane 1, alginate disaccharide (10 μg); lane 2, alginate trisaccharide (10 μg); lane 4, alginate disaccharide (10 μg) prepared by acid hydrolysis; lane 5, alginate trisaccharide (10 μg) prepared by acid hydrolysis. Tri, Di, and Mono indicate the unsaturated tri-, di-, and monosaccharides from alginate, respectively.