Figure 4.

The miR-563 Exo inhibits A549 cells proliferation and promotes apoptosis. (a) The changes in cell viability of A549 incubated with miR-563 Exo for 0, 24, 48, and 72 h was evaluated by CCK-8 assay. (b) EdU staining results of the incubation of A549 cells with exosomes. Scale bar = 100 nm. (c) Flow cytometric detection of apoptosis. (d) The protein expression level of Bax, cleaved caspase-3, cleaved caspase-9, and Bcl-2 evaluated by western blot. The values were presented as mean ± SD (n = 3). ^∗∗∗P < 0.001 versus the unloaded Exo group.