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. 2022 Aug 16;1(4):pgac160. doi: 10.1093/pnasnexus/pgac160

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© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Visualizing the fresh wholemount retina by SHG. (a) Experimental configuration for detecting transmitted SHG. (b) Multiphoton imaging of the CAG-H2B-EGFP retina (10) (an axial projection of z-stack images). The plexiform and nuclear layers are segregated by SHG (gray scale) and EGFP (green), respectively. Vertical SHG processes are also visible (arrows). (c) Volumetric rendering (371 × 371 × 186 µm³). (d) The segmented layers (lateral projections): ONL, the outer nuclear layer; OPL, the outer plexiform layer; INL, the inner nuclear layer; and IPL, the inner plexiform layer. Scale bars, 30 µm.