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. 2022 Aug 16;1(4):pgac160. doi: 10.1093/pnasnexus/pgac160

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© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 7. — Retinal SHG imaging is tunable to longer wavelengths for safety. (a) SHG imaging at = 800 and 1150 nm. Scale bars, 20 µm. (b and c) Light-induced changes evaluated by autofluorescence and SHG, respectively. The postirradiation images are the 10^th of z-stacks acquired every 5 min (i.e., t = 50 min). (d) SHG kymographs of the retina under continuous illumination. Axial swelling at 800 nm (arrowheads) but not at 950 nm.

Inline graphic — Retinal SHG imaging is tunable to longer wavelengths for safety. (a) SHG imaging at = 800 and 1150 nm. Scale bars, 20 µm. (b and c) Light-induced changes evaluated by autofluorescence and SHG, respectively. The postirradiation images are the 10^th of z-stacks acquired every 5 min (i.e., t = 50 min). (d) SHG kymographs of the retina under continuous illumination. Axial swelling at 800 nm (arrowheads) but not at 950 nm.