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. 2022 Apr 4;40(9):1378–1387. doi: 10.1038/s41587-022-01256-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Split orientation of T7-DdCBE and its target spacing region. Each spacing region contains TC repeats within the top strand (left, solid line) or bottom strand (right, dashed line). Lengths of spacing regions ranged from 12 bp to 18 bp. b–h, Editing efficiencies mediated by canonical DdCBE (purple), DddA6-containing DdCBE (red) and DddA11-containing DdCBE (blue) are shown for each cytosine positioned within the spacing region length of 12 bp (b), 13 bp (c), 14 bp (d), 15 bp (e), 16 bp (f), 17 bp (g) and 18 bp (h). Subscripted numbers refer to the positions of cytosines in the spacing region, counting the DNA nucleotide immediately after the binding site of TALE3 as position 1. Editing efficiencies associated with the top and bottom strand are shown as solid and dashed lines, respectively. Mock-treated cells contained only the library of target plasmids (gray). For b–h, values and error bars reflect the mean ± s.d. of n = 3 independent biological replicates. i, Approximate editing windows for canonical (purple), DddA6 (red) and DddA11 (blue) variants of T7-DdCBE containing the G1397 split. The length of each colored line reflects the approximate relative editing efficiency for each DddA variant.