Figure 7.

In vitro cell invasion in granular hydrogels. a) Schematic overview of embedding multicellular spheroids (HUVECs + hMSCs) in a fragmented granular hydrogel, with or without the addition RGD. b) Representative confocal microscopy images of F-Actin staining, showing spheroid outgrowth into granular hydrogels (top: control, bottom: adhesive), without RGD (left) and with RGD (right) after 3 days. c) Quantification of spheroid coverage area by percent (top) and absolute area in μm² (bottom). Data is presented as mean ± standard deviation, with a sample size of n ≥ 3. Statistical analysis performed using a one-way ANOVA. ns = no significance, **p<0.01.