TABLE 4.
Specificity of DNA Pol IV mutator activity
| Change | Mutation frequency (10−5)a
|
|||||
|---|---|---|---|---|---|---|
| AB1157/pWKS30 | AB1157/pYG782 | Ratiob | YG2237/pWKS30 | YG2237/pYG782 | Ratiob | |
| Base substitutions | ||||||
| G:C to T:A | 2.6 (47) | 8.2 (6) | 3.1 | 1.6 (8) | 3.7 (1) | 2.3 |
| G:C to A:T | 0.2 (4) | 4.1 (3) | 20 | 2.4 (12) | 7.3 (2) | 3 |
| A:T to T:A | 0.1 (2) | <12c | ||||
| A:T to G:C | 0.1 (2) | 12.3 (9) | 123 | 1.6 (8) | 44 (12) | 27.5 |
| A:T to C:G | 9.5 (7) | >170c | 29.4 (8) | >147c | ||
| G:C to C:G | 6.85 (5) | >121c | 3.7 (1) | >18c | ||
| Frameshifts | ||||||
| +1 in runs | 1.6 (28) | 4.1 (3) | 2.6 | 6.9 (35) | 22 (6) | 3.2 |
| −1 in runs | 0.3 (6) | 71 (52) | 237 | 1.8 (9) | 136 (37) | 85 |
| −1 in nonruns | 0.06 (1) | 2.7 (2) | 45 | 0.2 (1) | 3.7 (1) | 18.5 |
| Othersd | 0.1 (2) | 2.7 (2) | 27 | (0) | (0) | |
| Total | 5 (92) | 121 (89) | 14 (73) | 250 (68) | ||
a
Calculated using the corresponding cII mutation frequency presented in Table 1 and the relative frequency of each specific mutation recovered in the related spectrum. AB1157, wild-type strain; YG2237, mutS strain; pWKS30, empty vector; pYG782, dinB-carrying vector. Numbers of observed events are given in parentheses.
b
Ratio of mutation frequency in the strain harboring pYG782 to that in the strain harboring pWKS30.
c
Default value calculated on the basis of one observed event.
d
Insertion, 2-bp deletion, or addition.