Figure 5.

Photocrosslinking molded or printed cell-laden amide microgel scaffolds enables long-term stability for 2.5D culture. A–B) Annealing molded microgels maintained scaffold architecture without decreasing cell viability. C) Maximum intensity projection of confocal z-stack demonstrating cell spreading and high viability after 3 days of culture. D) Microgels are easily printed into 3D structures with high fidelity before annealing. Inset: macroscopic view of printed microgel filament. Bin widths for all histograms are ~0.01. Scale bars = 500 μm. Live and dead cells were respectively stained with calcein AM (green) and ethidium homodimer (red).