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. Author manuscript; available in PMC: 2023 Sep 1.
Published in final edited form as: Small. 2022 Jun 22;18(36):e2200951. doi: 10.1002/smll.202200951

Figure 6.

Figure 6.

MMP-labile microgels enable on-demand release of cells in 2.5D culture. A) Maximum intensity projection of confocal z-stack demonstrating cell spreading and B) high viability after 7 days of culture in MMP-labile microgel scaffolds. C) Confocal image of cells passaged from 7 days of 2.5D culture after 3 days of 2D culture. D) Cell viability remains >80% with collagenase D treatment both 1 hour and 3 days following cell release from MMP-labile scaffolds. Scale bars = 250 μm. Live and dead cells were respectively stained with calcein AM (green) and ethidium homodimer (red).