Figure 1.

PBM irradiation and experimental schedule. (A) Beam profiling of PBM using a spectrophotometer. An 830 nm laser with specific narrow wavelength was used to irradiate the SE mouse model. (B) Experimental setup for treatment with PBM. After the hair on the head was depilated, the mouse was placed in the 50 ml tube with a hole in the head position to fix the movement, and then an 830 nm laser was used for irradiation for 12 min at a distance of 5 cm with a power of 50 mW/cm². (C) The scalp was irradiated with an area of 14.01 mm², and laser transmission were measured by power-meter detector. (D) 830 nm laser was irradiated at 4 h after SE-induction only once as 36 J or during 5 days as total 180 J. Then, brains were collected for the epifluorescence analyses on days 7, 14, or 21. This figure was generated with Microsoft Visio version 2016 (Microsoft, Redmond, WA, USA).