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. 2022 Sep 10;13(9):781. doi: 10.1038/s41419-022-05223-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A The mRNA levels of the 15 genes with relative high fold change were verified by RT-qPCR. B The effect of ZNF276 knockdown on the mRNA expression of CYP1B1. C Western blot analysis of the effect of ZNF276 overexpression and knockdown on CYP1B1 expression. D Dual-luciferase reporter gene assay showing ZNF276 overexpression promoted luciferase activity of CYP1B1 promoter. E Dual-luciferase reporter gene assay showing inhibition of ZNF276 decreased luciferase activity of CYP1B1 promoter. F Construction of truncated fragments of ZNF276 for investigating the binding site of ZNF276 on CYP1B1 promoter. G Dual-luciferase reporter gene assay measuring the CYP1B1 promoter luciferase activity driven by different truncated fragment of ZNF276. H Construction of truncated fragments of CYP1B1 promoter for investigating the ZNF276 binding site. I Dual-luciferase reporter gene assay measuring the truncated CYP1B1 promoter luciferase activity in ZNF276 overexpressing and control cells. J ChIP-PCR assay demonstrated a direct binding of ZNF276 at the CYP1B1 promoter. *p < 0.05; **p < 0.01; ***p < 0.001.