Fig. 3.

MiR-590-3p targeted VEGFA regulates its expression (A–B) agomir-590-3p/antagomir-590-3p was transfected into ADSCs. VEGFA levels in cells were determined using Western blot (A). VEGFA levels in the culture medium were determined by ELISA assay (B). N = 3; ∗P < 0.05, compared with agomir-NC group; ##P < 0.01, compared with antagomir-NC group (C) MiR-590-3p and VEGFA expressions were detected in ADSCs bound to the Ago2 antibody or IgG by RIP assay. N = 3; ∗∗P < 0.01, compared with IgG group (D–E) agomir-590-3p and/or si-VEGFA were transfected into ADSCs and the VEGFA levels in cells and culture medium were examined using Western blot (left) and ELISA assay (right). N = 3; ∗P < 0.05, ∗∗P < 0.01, compared with antagomir-NC + si-NC group; ##P < 0.01 compared with antagomir-NC + si-VEGFA group.