FIG. 6.

Analysis of AdpA-H binding to the region downstream of the adsA promoter by DNase I footprinting assays. Probe sigS1H for the antisense strand was prepared by PCR with primers sigS1H-F and sigS1H-R (Fig. 3C). Probe sigFP for the sense strand was similarly prepared with primers sigFP-F and sigFP-R. The antisense strand from positions +7 to +41 with respect to the transcriptional start point of adsA is protected, and an additional sequence from positions −1 to +6 is also weakly protected. The sense strand from positions +12 to +46 is protected, and a sequence from positions +47 to +53 is also weakly protected. An inverted repeat within the AdpA-H-binding site is indicated between the both strands, together with the transcriptional start point of adsA.