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In vitro transcription of the bldMp1 promoter by reconstituted EςBldN holoenzyme. Transcripts were generated from templates 1 and 2 (see Materials and Methods) using core RNA polymerase alone or core enzyme plus ςBldN. The expected sizes of the runoff transcripts from the bldMp1 promoter were 104 nucleotides (template 1) and 78 nucleotides (template 2). The size markers (M) were a 32P-end-labeled HinfI digest of ΦX174.
