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. 2022 Aug 28;56:102449. doi: 10.1016/j.redox.2022.102449

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Downregulation of Nrf2 impairs the survival and anigogenic function of Healthy-EPCs, while upregulation of Nrf2 rescues the survival and angiogenic function of DM-EPCs. Healthy-EPCs were transfected with lentivirus carrying nonsense shRNA (Ctrl-shRNA) or Nrf2 shRNA (Nrf2-shRNA) and DM-EPCs were transfected with lentivirus carrying Nrf2 (Lv-Nrf2) or control vector (Lv-Ctrl). (A-B) The efficiency of Nrf2 knockdown or upregulation was determined by Western blot and qRT-PCR, and the quantitative data was normalized by the average of the respective control group for each protein; β-actin was used as a loading control. (C) The survival abilities were determined by Annexin V/PI staining, and the quantitative data was expressed as the percentage of AnnexinV⁺/PI^– cells. (D) Angiogenic function of EPCs was evaluated by a Matrigel tube formation assay, and the quantitative data was normalized by the average tube length of the respective control group. n = 4 per group. Data shown in graphs represents the means ± SD. *p < 0.05, vs Ctrl-shRNA or Lv-Ctrl.