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. 2022 Aug 28;56:102449. doi: 10.1016/j.redox.2022.102449

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 3 — Nrf2 regulates the angiogenic function of mouse EPCs. WT-EPCs were transfected with lentivirus carrying Ctrl-shRNA or Nrf2-shRNA and db/db-EPCs were transfected with lentivirus carrying Lv-Ctrl or Lv-Nrf2. Within 1 h after hind limb ischemia (HLI) surgery, db/db mice (FVB background) were transplanted with different types of EPCs that have a same genetic background. (A) The time-course of blood perfusion before (BEF) and after HLI surgery was monitored by a Pericam Perfusion Speckle Imager, and the blood perfusion was quantified by Image J and expressed as the percentage of the perfusion relative to the collateral nonischemic limb. Transverse sections of ischemic gastrocnemius (B) and soleus muscle (C) were stained with Alexa Fluor® 594 conjugated isolectin to enumerate isolectin-stained cell number as a proxy of capillary density. Capillary density was expressed as isolectin⁺ capillaries per muscle fiber. n = 6 mice per group. Data shown in graphs represents the means ± SD. *p < 0.05, vs Ctrl-shRNA or Lv-Ctrl.