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. 2022 Aug 28;56:102449. doi: 10.1016/j.redox.2022.102449

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Nrf2 expression affected the protein expression of mitochondrial fusion and fission related proteins but not mRNA expression. Healthy-EPCs were transfected with lentivirus carrying Ctrl-shRNA or Nrf2-shRNA and DM-EPCs were transfected with lentivirus carrying Lv-Ctrl or Lv-Nrf2. The expression of mitochondrial fusion (A-B) and fission (C-D) related molecules at protein and mRNA levels were determined by Western blot and q-RT-PCR, respectively; the quantitative data was normalized by the average of the respective control for each molecule, and β-actin was used as a loading control. n = 4 per group. Data shown in graphs represents the means ± SD. *p < 0.05, vs Ctrl-shRNA or Lv-Ctrl.