Fig. 1.

A The schematic diagram of expression cassette of pPZP211: CTB-TM-1. Genetic components included are: PGluB-4, promoter of glutelin gene GluB-4; CTB, Cholera non-Toxin B subunit; Furin, Furin cleavage site; TM-1, TM-1 protein of Mycoplasma gallisepticum (MG); KDEL, Endoplasmic reticulum retention signal; Tnos, termination of the nopaline synthase gene from Agrobacterium tumefaciens; CaMV 35S, promoter of 35S RNA from Cauliflower Mosaic Virus; KanR, Kanamycin resistant gene; CaMV Poly(A), Cauliflower Mosaic Virus polyadenylation signal; LB and RB, left and right border of T-DNA repeat, respectively. Arrows indicate PCR and sequencing primers. B characterization of genomic integration of pPZP211: CTB-TM-1 cassette in different transgenic lines by PCR using primers (CTB-F and TM-1-R). C Validation of recombinant CTB-TM-1 expression in three lines by Western blot with anti-CTB antibodies. D quantification of recombinant CTB-TM-1 expression in C. E GM1 ELISA detecting the folding of CTB-TM-1 and its binding with GM1 receptor. *, P < 0.05, **, P < 0.01, ***, P < 0.001