Figure 3.

miR-424 and miR-503 act as tumor suppressors in vitro and in vivo

(A) Cell viability after the overexpression of miR-424 and miR-503 in adherent SKOV3 cells. (B) Colony formation was determined using crystal violet staining 14 days after the miR-424 and miR-503 overexpression in adherent SKOV3 cells. Representative images of the (C) migration assay after miR-424/503 overexpression in adherent SKOV3 cells. Scale bar: 200 μm. (D) Incidence and size of subcutaneous tumors in nude mice at 14 days after injection with control or miR-424/503-overexpressing SKOV3 cells. The graphs show significantly decreased tumor size in mice injected with miR-424/503-overexpressing cells compared with that in mice injected with control cells. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared with controls by unpaired two-tailed Student’s t test or two-way ANOVA with Bonferroni’s multiple comparison test. Error bars, standard error of the mean. (E) Therapeutic efficacy of lentivirus-expressing miR-424/503 on established xenografts compared with the Virus Scramble xenografts. ∗∗p < 0.01, ∗∗∗p < 0.001 by mixed model for repeated data with Bonferroni’s multiple comparison test.