Figure 4.

miR-424 and miR-503 inhibit cancer stem cell-like properties in ovarian cancer cells

The sizes of spheroids after the overexpression (A) or inhibition (B) of miR-424/503 in primary ovarian tumor cells and SKOV3 cells. Scale bars: 100 μm. (C) Cell viability after the overexpression of miR-424 and miR-503 in SKOV3 spheroids. (D and E) Fluorescence-activated cell sorting analysis of the CD133- and ALDH1⁺ subpopulation in primary ovarian cancer cells and SKOV3 cells after miR-424/503 overexpression. (F) Representative images of SKOV3 spheroids, and WEE1 protein expression levels after concurrent miR-424/503 and WEE1 overexpression. Scale bar: 250 μm. ∗∗p < 0.01, ∗∗∗p < 0.001 compared with controls by unpaired two-tailed Student’s t test or one-way ANOVA with Bonferroni’s multiple comparison test. Error bars, standard error of the mean.