Figure 6.

NANOG upregulates WEE1 expression by downregulating miR-424 and miR-503 expression

(A–C) Protein and mRNA levels of NANOG in SKOV3 and OVCAR8 adherent cells and spheroids. (D) Mature miR-424 and miR-503 expression in response to NANOG overexpression. (E and F) WEE1 protein expression in response to NANOG overexpression. (G and H) WEE1 protein expression in response to miR-424/503 overexpression with concurrent NANOG overexpression. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared with controls by unpaired two-tailed Student’s t test or one-way ANOVA with Bonferroni’s multiple comparison test. Error bars, standard error of the mean.