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Characterization of recombinant, purifiedwild typeCYP8B1. (A), SDS-PAGE, (B) UV-visible absorbance spectrum, indicating heme incorporation with a Soret λmax at 420 nm, (C) reduced carbon monoxide difference spectrum, and (D) reduced carbon monoxide difference spectrum in the presence of 7α-hydroxycholest-4-en-3-one. CYP8B1, cytochrome P450 8B1.
