Table 1.
Chronic unpredictable stress protocol schedule
| Dark phase (red light) |
Light phase |
|||
|---|---|---|---|---|
| ∼ 8 am | ∼ 12 pm | ∼ 4 pm | ∼ 8 pm | |
| DAY 1 | INITIAL ACCLIMATIZATION | |||
| DAY 2 | INITIAL ACCLIMATIZATION | |||
| DAY 3 | INITIAL ACCLIMATIZATION | |||
| DAY 4 | INITIAL ACCLIMATIZATION | |||
| DAY 5 | INITIAL ACCLIMATIZATION | |||
| DAY 6 | INITIAL ACCLIMATIZATION | |||
| DAY 7 | INITIAL ACCLIMATIZATION | |||
| DAY 8 | HANDLING | |||
| DAY 9 | HANDLING | |||
| DAY 10 | HANDLING | |||
| DAY 11 | HANDLING | |||
| DAY 12 | HANDLING | |||
| DAY 13 | HANDLING | |||
| DAY 14 | HANDLING | |||
| DAY 15 | SPT pretest | |||
| DAY 16 | SPT | SPT BS | BW | RAT |
| DAY 17 | SS | RS | RAT | |
| DAY 18 | TSS | SS | RAT | |
| DAY 19 | RS | TSS | RAT | |
| DAY 20 | SS | RS | RAT | |
| DAY 21 | TSS | SS | RAT | |
| DAY 22 | RS | TSS | RAT | |
| DAY 23 | SS | RS | RAT | |
| DAY 24 | TSS | SS | RAT | |
| DAY 25 | RS | TSS | RAT | |
| DAY 26 | SS | RS | RAT | |
| DAY 27 | TSS | SS | RAT | |
| DAY 28 | RS | TSS | RAT | |
| DAY 29 | SS | RS (∼ 3 pm) | ||
| DAY 30 | SPT | SPT BS | FST, BW | |
| DAY 31 | ISOLATION OF THE TISSUES FOR ANALYSIS | |||
SPT – sucrose preference test, SPT BS - bottles switch sides after 4 h of testing, SS – social defeat stress, RS - restraint stress, RAT - exposure to a predator (rat), TS - tail suspension stress, FST – forced swim test, BW - body weight measurement.