Figure 3.

Fibrinolysis in contracted versus uncontracted clot simulations. Model contracted (A, left) and uncontracted (A, right) clots are built to mimic the experimental clots. Three independent simulations were conducted for both contracted and uncontracted clots with t-PA added at the clot’s edge, and degradation of the clot was measured by the fraction of fibrin remaining in the clot over the course of time (B). The experimental rate of clot degradation (contracted versus impaired contraction with blebbistatin, a myosin inhibitor (22)), and modeling rate of clot degradation (90RCT versus 0RU) are compared (C); experimental degradation is measured as percentage of fibrin degradation products (% FDP) per hour; modeling degradation was measured in fraction of fibrin degraded per second (ff/s), and was determined by calculating the slope of the linear portion of (B). Time to 50% degradation (D) and 90% degradation (E) were analyzed. Data are represented as mean $\pm$ SEM. Data were analyzed using a one-way ANOVA followed by a Tukey’s multiple comparison test. ^∗∗∗∗p < 0.0001. To see this figure in color, go online.