Table 3.
Summary of FITC-derivatized dextrans used in pore estimation assays
Compound | MW (kDa) | Stokes radius (Å) | Stokes radius (nm) | Hydro. diameter (nm) | Solubility (mg/mL) | Concentration (mM) | Leakage (at EC80) |
---|---|---|---|---|---|---|---|
FD10S | 10 | 23 | 2.3 | 4.6 | above 25 | 2.5 | not tested |
FD40S | 40 | 45 | 4.5 | 9 | 25 | 2.5 | yes |
FD70S | 60–76 | 60 (70S) | 6.0 | 12 | 25 | 2.5 | yes |
FD250S | 250 | 105 | 10.5 | 12–21 | 25 | 2.5 | yes |
FD500S | 500 | 75 | 7.5 | 15 | 25 | 2.5 | not tested |
Hydro. diameter, hydrodynamic diameter.