Dendritic activity in networks causes a reduction in filament length and increases monomer replacement by treadmilling. (a and b) The plot of (a) filament length and (b) network treadmilling rate of networks at various Arp2/3 concentrations. (a) Solid lines and shaded areas represent the mean and standard deviation, respectively, of filament lengths from six replicates. (b) Mean treadmilling rate of the network is plotted as a bar graph, with error bars representing standard deviation. Networks' approach to steady state was quantified based on time invariance of mean treadmilling rate. As the treadmilling rate stabilized around 1500 s, data from the last 500 s of trajectories (six replicates, sampling frequency = 5 s) was employed in (a) and (b). To see this figure in color, go online.