Figure 7.

Analysis of domain dynamics reveals the underlying mechanism of F-actin organization into high-density actin domains. (a) The cartoon rendering describes elementary transition mechanisms showing F-actin domains in red, with brown regions representing low-density actin background. (aI–aIV) Four different transition mechanisms in a reference snapshot that affect the number of clusters in the following snapshot. (b) Pie charts showing the percentage of transitions observed throughout the trajectory at different Arp2/3 concentrations. The size of the pie chart is proportional to the total number of transitory events. (Fig. S6 shows data from entire trajectory in addition to specific time ranges as bar graph along with the standard deviation) (c and d) Estimation of (c) drift and (d) drift-corrected diffusion coefficients (bottom row) at various cluster sizes from trajectories of high Arp2/3 concentrations, 25 (left column) and 50 nM (right column) show how the instantaneous change (drift) and spread in number of domains (diffusion coefficient) depends on the number of domains present in the snapshot. (b–d) Entire trajectories of all replicates corresponding to each of the Arp2/3 concentrations are employed to compute these results. To see this figure in color, go online.