FIG. 4.

Phosphomonoester region of the ³¹P NMR spectrum of an ethanol extract from T. zilligii. The cell suspension was incubated at 75°C with 15 mM glucose for 2 h and extracted for 30 min with 70% ethanol. The final pH of the sample was 6.9. The spectrum was acquired on a Bruker DRX500 spectrometer using a 10-mm quadruple nucleus probe head (repetition delay, 1.8 s; pulse width, 16 μs, corresponding to 55° flip angle; proton decoupling during acquisition only). Assignments were made by spiking the extracts with the pure compounds at pH 6.9 and 8.2. Abbreviations: X-5-P, xylulose-5-phosphate; G-6-P, glucose-6-phosphate.