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. 2000 Aug;182(16):4632–4636. doi: 10.1128/jb.182.16.4632-4636.2000

TABLE 3.

Percentage of 13C labeling on the fermentation products derived from the metabolism of [13C]glucose by cell suspensions of T. zilligii grown on glucosea

Substrate Value for product:
Acetate
Formate
Total amt (μmol/mg of protein) 13CH3COO (%) CH313COO (%) Total amt (μmol/mg of protein) H13COO (%)
[1-13C]glucoseb 0.88 15 d 0.05 66
[2-13C]glucosec 1.21 ± 0.38 7 ± 1 15 ± 2 0.09 ± 0.05
a

Cell suspensions were incubated with 15 mM glucose at 75°C for 2 h. Products were quantified by proton NMR using formate as a standard. Percentages of 13C labeling were calculated by integration of the respective resonances in the 1H spectra. The natural abundance of 13C (1.1%) was subtracted. 

b

Result from a single experiment. 

c

Average values for two independent experiments. 

d

—, not detected.