TABLE 3.
Substrate | Value for product:
|
||||
---|---|---|---|---|---|
Acetate
|
Formate
|
||||
Total amt (μmol/mg of protein) | 13CH3COO− (%) | CH313COO− (%) | Total amt (μmol/mg of protein) | H13COO− (%) | |
[1-13C]glucoseb | 0.88 | 15 | —d | 0.05 | 66 |
[2-13C]glucosec | 1.21 ± 0.38 | 7 ± 1 | 15 ± 2 | 0.09 ± 0.05 | — |
Cell suspensions were incubated with 15 mM glucose at 75°C for 2 h. Products were quantified by proton NMR using formate as a standard. Percentages of 13C labeling were calculated by integration of the respective resonances in the 1H spectra. The natural abundance of 13C (1.1%) was subtracted.
Result from a single experiment.
Average values for two independent experiments.
—, not detected.