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Amyrin titers in the engineered Y. lipolytica with mutated CrMAS in YPD. A Effects of different alanine point mutation on the enzymatic activity of CrMAS. B Expression of the best performing mutant CrMAS L323A in strains metabolically engineered with enhanced precursor supplies. Control strains: Po1g ΔKU70 and Po1g KC. Amyrins were quantified after 5 days of cultivation in shake flasks. All values presented are the mean of three biological replicates ± standard deviation
