Table 2.
List of plasmids employed in this study
| Plasmid | Description | Source |
|---|---|---|
| pYLEX1 | Y. lipolytica integrative plasmid, pHP4D-tXPR2, leu2, Apr | 40 |
| pUCCrMAS | codon-optimized (naturally occurring) crmas, Apr | Beijing Genomics Institute |
| pYLCrMAS | pHP4D-crmas-tXPR2, leu2, Apr | This study |
| pYLE1 | pHP4D-erg1-tXPR2, leu2, Apr | This study |
| pYLE9 | pHP4D-erg9-tXPR2, leu2, Apr | This study |
| pYLE20 | pHP4D-erg20-tXPR2, leu2, Apr | This study |
| pYLH | pHP4D-hmg1-tXPR2, leu2, Apr | This study |
| pYLC-240A | pHP4D-crmasP240A-tXPR2, leu2, Apr | This study |
| pYLC-243A | pHP4D-crmasF243A-tXPR2, leu2, Apr | This study |
| pYLC-251A | pHP4D-crmasP251A-tXPR2, leu2, Apr | This study |
| pYLC-323A | pHP4D-crmasL323A-tXPR2, leu2, Apr | This study |
| pYLC-324A | pHP4D-crmasL324A-tXPR2, leu2, Apr | This study |
| pYLC-323A/324A | pHP4D-crmasL323A/L324A-tXPR2, leu2, Apr | This study |
| pYLC-328A | pHP4D-crmasL328A-tXPR2, leu2, Apr | This study |
| pYLC-331A | pHP4D-crmasF331A-tXPR2, leu2, Apr | This study |
| pYLC-341A | pHP4D-crmasC341A-tXPR2, leu2, Apr | This study |
| pYLCE1 | pHP4D-crmas-tXPR2, pHP4D-erg1-tXPR2, leu2, Apr | This study |
| pYLCE9 | pHP4D-crmas-tXPR2, pHP4D-erg9-tXPR2, leu2, Apr | This study |
| pYLCE20 | pHP4D-crmas-tXPR2, pHP4D-erg20-tXPR2, leu2, Apr | This study |
| pYLCH | pHP4D-crmas-tXPR2, pHP4D-hmg1-tXPR2, leu2, Apr | This study |
| pYLCHE1 | pHP4D-crmas-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg1-tXPR2, leu2, Apr | This study |
| pYLCHE9 | pHP4D-crmas-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg9-tXPR2, leu2, Apr | This study |
| pYLCHE20 | pHP4D-crmas-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg20-tXPR2, leu2, Apr | This study |
| pYLCE1E9 | pHP4D-crmas-tXPR2, pHP4D-erg1-tXPR2, pHP4D-erg9-tXPR2, leu2, Apr | This study |
| pYLCE1E20 | pHP4D-crmas-tXPR2, pHP4D-erg1-tXPR2, pHP4D-erg20-tXPR2, leu2, leu2, Apr | This study |
| pYLCE9E20 | pHP4D-crmas-tXPR2, pHP4D-erg9-tXPR2, pHP4D-erg20-tXPR2, leu2, leu2, Apr | This study |
| pYLCHE9-323A | pHP4D-crmasL323A-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg9-tXPR2, leu2, Apr | This study |
| pYLCHE20-323A | pHP4D-crmasL323A-tXPR2, pHP4D-hmg1-tXPR2, pHP4D-erg20-tXPR2, leu2, Apr | This study |