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. 2022 Sep 9;14:50. doi: 10.1186/s11689-022-09461-x

Fig. 2.

Fig. 2

Luciferase assay vs cis-regulatory MPRA. A Luciferase assay measures light emitted by a reporter gene, luciferase, driven by a regulatory element. B In a canonical cis-regulatory MPRA, the regulatory element drives the RNA expression of the unique barcodes. Transcriptional activity is quantified as barcode transcription (via RNA-seq of the barcodes) normalized to initial input of barcodes (via DNA-seq of the barcodes). Thousands of cis-regulatory elements (CREs) can be tested in the same experiment