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. 2022 Sep 10;15:33. doi: 10.1186/s13072-022-00466-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Bisulfite Pyrosequencing of F1 tissues. Bisulfite pyrosequencing detected heritable changes at A Pxylp1 in F1 sperm; B Mtss1l in F1 hippocampus; and C Mtss1l in F1 NAc. CpG sites labeled with “*” correspond to those identified via WGBS in the LE v C or EE v C datasets. Black, control offspring; blue, EE offspring; green, LE offspring. Two-factor ANOVA (factor 1 = exposure status, factor 2 = CpG site) were significant for all genes. Significance indicators on graphs represent post hoc two-tailed t-tests. ^#p = 0.05, ^*p < 0.05, ^**p < 0.01