Figure 5.
The role of peripheral oscillators located in epithelial glia in sleep regulation in LD12:12. A, B, Clock disruption in the epithelial glia (TubGal80ts;netB>cycΔ24) affects sleep during the day and night comparing with both parental strains (TubGal80ts;netB-Gal4/+, UAS- cycΔ24/+). C, D, Per silencing in the epithelial glia affects sleep time during the day. E, Adult-specific neurotransmission disruption in epithelial glia (TubGal80ts;netB>ebonyRNAi) affects sleep amount during the day. Statistically significant differences are marked with asterisks: *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001. Detailed statistics are provided in Table 2. F, Temperature-dependent clock disruption in epithelial glia does not affect daily changes in the complexity of sLNv neurons terminals (differences between time points are statistically significant with p = 0.0061 for control, p = 0.0031 for experimental group), but it decreases amplitude of this oscillations. Means ± SE.