Table 2.

Comparative amino acid substitutions of the isolates from Canada and Japan to H5N8 A/Gyrfalcon/Washington/41,088-6/2014. Putative antigenic positions were annotated based on H5 numbering. Amino acid residues 185, 195, and 198 form antigenic site B; amino acids 210 and 268 form antigenic sites D and C, respectively.

	Amino acid residues
Viruses	14	94	104	114	119	141	185	195	198	210	223	236	268	269	282	325
A/Gyrfalcon/Washington/41,088-6/2014_H5N8	K	T	L	T	R	S	A	D	V	V	S	N	E	M	I	R
A/Bald_Eagle/Canada/OTH-33-36/2022_H5N1	E	S		I	K	P	E	T	I		R	D	G	V	V
A/white-tailed_eagle/Hokkaido/R22/2022_H5N1	E	S		I	K	P	E	T	I		R	D	G	V	V	K
A/Chicken/BC/FAV-0228/2022_H5N1	E	S	M	I	K	P	E	T	I	A	R	D	G	V	V	K