Table 1.
Gene | Primers’ first reaction | PCR product length | Primers’ second reaction | PCR product length |
---|---|---|---|---|
SSU rRNA |
Forward(F1): 5′-TTCTAGAGCTAATACATGCG-3′ Reverse(R1): 5′-CCCATTTCCTTCGAAACAGGA-3′ |
1319 bp |
Forward (F2): 5′-GGAAGGGTTGTATTTATTAGATAAAG-3′ Reverse (R2): 5′- AAGGAGTAAGGAACAACCTCCA-3′ |
834 bp |
GP60 |
Forward (F1): 5′-ATAGTCTCCGCTGTATTC-3′ Reverse (R1): 5′-GGAAGGAACGATGTATCT-3′ |
921 bp |
Forward(F2): 5′-TCCGCTGTATTCTCAGCC-3′ Reverse (R2): 5′-GCAGAGGAACCAGCATC-3′ |
887 bp |
GP60- Canis |
Forward (F1): 5′-ATACTCTGGTCTCCCGTTT-3′ Reverse (R1): 5′-GTACTCGGAAGCGGTGTA-3′ |
750 bp |
Forward (F2): 5′-AAGGCGCCTCACTCATT-3′ Reverse (R2): 5′-TCAGTTAGATATCACCCATTAA-3′ |
700 bp |
bp, base pairs