Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 10;9:558. doi: 10.1038/s41597-022-01546-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Examples of OPT and LSFM based data visualization and quantification. The datasets included in this data descriptor can be used for a multitude of assessments pertinent to STZ-induced diabetes in mice. This includes a range of quantitative and 3D-spatial analyses (volumes, signal intensities, numbers, shapes, distribution patterns, etc.) of insulin and GLUT2 stained islets of Langerhans subject to different modes of STZ administration and at different time points. (a,b) Images of maximum intensity projections based on pancreatic volumes from control C57BL/6 mice showing the distribution of insulin labelled islets of Langerhans (red) in the pancreatic splenic lobe imaged by OPT (a) and high-resolution LSFM image (b). (c,d) The same specimen as in (a,b) in which the intensity of the signal intensity has been colour coded. Blue colour corresponds to low and red to high signal intensity, respectively. (e) Graph illustrating the possibility to study statistically assess spatial distribution patterns subject to STZ treatment. (f) Graph illustrating the possibility to statistically assess staining intensities of islets of different size categories subject to STZ administration. Data in (e,f) is based on pancreata 2 weeks post SHD administration). Scale bar in (c,d) corresponds to 1 mm and 200 µm in (a,b) respectively. Data is presented as averages and error bars represent SEM. Significance in graph (f) was tested using a One-way ANOVA. * represents P ≤ 0.05, ** represents P ≤ 0.01 and *** represents P ≤ 0.001.