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. 2022 Sep 10;9:558. doi: 10.1038/s41597-022-01546-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Schematic illustration of experimental setup and data generation. (a) Animals were subjected to different STZ treatment regimens and the pancreata were collected over 3 weeks. The pancreata were subsequently divided into the three primary lobular compartments (splenic lobe (SL), duodenal lobe (DL) and gastric lobe (GL)) prior to whole mount immunohistochemistry (insulin-594 and GLUT2-680), agarose embedding and tissue clearing for OPT-scanning. dataset 1 consist of control, single high dose (SHD) and multiple low doses (MLD) at 1-, 2- and 3-weeks post STZ administration (n = 5, n = 3–5 and n = 3–5, respectively per time point). (b) Animals were subjected to a single high dose STZ treatment and recovered from hyperglycemia by transplanting islet of Langerhans to the anterior chamber of the eye (SHD + Tx). dataset 2 consists of data from pancreatic splenic lobe (SL) only from control samples (n = 7), SHD (n = 8) and SHD + Tx (n = 4) 4 weeks post STZ administration. (c) dataset 3 consists of LSFM image data and is generated based on a selection of representative samples from dataset 1.